Formation of Amyloid Fibrils In Vitro from Partially Unfolded Intermediates of Human γC-Crystallin
نویسندگان
چکیده
PURPOSE. Mature-onset cataract results from the formation of light-scattering aggregates of lens crystallins. Although oxidative or mutational damage may be a prerequisite, little is known of the initiation or nucleation of these aggregated states. In mice carrying mutations in -crystallin genes, a truncated form of -crystallin formed intranuclear filamentous inclusions within lens fiber cells. Previous studies have shown that bovine crystallins and human D-crystallin form amyloid fibrils under denaturing conditions in vitro. The amyloid fibril formation of human C-crystallin (H C-Crys) induced by low pH, together with characterization of a partially unfolded intermediate in the process were investigated.
منابع مشابه
Formation of amyloid fibrils in vitro from partially unfolded intermediates of human gammaC-crystallin.
PURPOSE Mature-onset cataract results from the formation of light-scattering aggregates of lens crystallins. Although oxidative or mutational damage may be a prerequisite, little is known of the initiation or nucleation of these aggregated states. In mice carrying mutations in gamma-crystallin genes, a truncated form of gamma-crystallin formed intranuclear filamentous inclusions within lens fib...
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